doi: 10.1007/978-1-4939-9048-1_15.
Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila
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- PMID: 30694496
- DOI: 10.1007/978-1-4939-9048-1_15
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Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila
Methods Mol Biol.
2019.
Abstract
Legionella utilizes specialized protein secretion machinery called the type IV secretion system encoded by dot/icm genes to modulate host cellular systems. We describe here the procedure to isolate the core complex of the Dot/Icm type IV secretion system of L. pneumophila based on detergent lysis of bacteria and ultracentrifugation. The isolated protein complex can be applied for biochemical and transmission electron microscopy analysis.
Keywords: Core complex; Electron microscopy; Legionella; Molecular structure; Nanomachine; Type IV secretion; Ultracentrifugation.
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