Aim: To evaluate the antibiofilm effect and esp gene downregulation of Enterococcus faecalis through nanozinc oxide fabricated on natural zeolite (NanoZnO/Ze). Materials & methods:Zeolite and NanoZnO/Ze materials were characterized by x-ray diffraction, x-ray fluorescence and field emission scanning electron microscopy coupled with energy dispersive x-ray. Atomic absorption spectroscopy was used to evaluate zinc release. E. faecalis biofilm formation and its esp gene expression were assessed under nanocomposite treatment.
Results: Spherical-shaped ZnO nanoparticles with an average size of 30 nm were dispersed on the zeolites surface. The leakage of cationic zinc from NanoZnO/Ze displayed a long lasting and considerable release content (p < 0.0001) compared with ZnO/Ze. NanoZnO/Ze effectively inhibited (p < 0.0001) biofilm formation and affected esp gene downregulation of E. faecalis.
Conclusion: Our results show that NanoZnO/Zeolite can potentiate against biofilm infections due to E. faecalis and possibly other pathogens.
Keywords: gene; FESEM; MBIC; NanoZnO; XRD; antibiofilm; dental; expression; natural zeolite; sustained release.