Surface exposure of the O-antigen in Klebsiella pneumoniae O1:K1 serotype strains

Microb Pathog. 1988 Aug;5(2):141-7. doi: 10.1016/0882-4010(88)90016-2.


Isogenic non-encapsulated (K-) mutants (O1:K-) were obtained from several different Klebsiella pneumoniae O1:K1 serotype strains. By employing K. pneumoniae bacteriophages FC3-1, FC3-2, and bacteriophage phi 1, the bacterial surface receptors of which, are the O1-antigen of lipopolysaccharide and the K1 capsular polysaccharide (K1) respectively, the K1 polysaccharide was found to completely cover the O1 lipopolysaccharide molecules in each of the O1:K1 strains examined. Exposure of the O-antigen at the cell surface was only observed after growth in the presence of sub-minimum inhibitory concentrations of antibiotics. The implications of these findings for the design of vaccines for the prevention of Klebsiella infections is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Bacterial / analysis*
  • Antigens, Surface / analysis*
  • Bacteriophages
  • Klebsiella pneumoniae / immunology*
  • Lipopolysaccharides / analysis*


  • Antigens, Bacterial
  • Antigens, Surface
  • Lipopolysaccharides