Monitoring ADP/ATP ratio in yeast cells using the fluorescent-protein reporter PercevalHR

Biosci Biotechnol Biochem. 2019 May;83(5):824-828. doi: 10.1080/09168451.2019.1574204. Epub 2019 Jan 31.


PercevalHR (Perceval High Resolution) is an artificially designed fluorescent protein, which changes its excitation spectrum based on the ADP/ATP ratio of the environment. Here we demonstrated that PercevalHR can be used for monitoring energy status of Saccharomyces cerevisiae cells, which are affected by diauxic shift and mitochondria inhibition, in a non-invasive and non-destructive manner.

Keywords: Fluorescent reporter: ATP: ADP; diauxic shift; yeast.

MeSH terms

  • Adenosine Diphosphate / metabolism*
  • Adenosine Triphosphate / metabolism*
  • Fluorescence
  • Luminescent Proteins / metabolism*
  • Mitochondria / metabolism
  • Saccharomyces cerevisiae / metabolism*


  • Luminescent Proteins
  • Adenosine Diphosphate
  • Adenosine Triphosphate