Single-Molecule FRET Methods to Study Glutamate Receptors

Douglas B Litwin; Ryan J Durham; Vasanthi Jayaraman

doi:10.1007/978-1-4939-9077-1_1

Single-Molecule FRET Methods to Study Glutamate Receptors

Methods Mol Biol. 2019:1941:3-16. doi: 10.1007/978-1-4939-9077-1_1.

Authors

Douglas B Litwin¹, Ryan J Durham¹, Vasanthi Jayaraman²

Affiliations

¹ Center for Membrane Biology, Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston, Houston, TX, USA.
² Center for Membrane Biology, Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston, Houston, TX, USA. Vasanthi.jayaraman@uth.tmc.edu.

Abstract

Single-molecule fluorescence energy transfer methods allow us to determine the complete structural landscape between the donor and acceptor fluorophores introduced on the protein of interest. This method is particularly attractive to study ion channel proteins as single-molecule current recordings have been used to study the function of these proteins for several decades. Here we describe the smFRET method used to study glutamate receptors.

Keywords: Fluorescence; Glutamate receptor; NMDA receptor; Single-molecule FRET.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Fluorescence
Fluorescence Resonance Energy Transfer / methods*
Humans
Receptors, Glutamate / metabolism*
Single Molecule Imaging / methods*

Substances

Receptors, Glutamate

Abstract

Publication types

MeSH terms

Substances

Grants and funding