Insights into the intestine immune of Marsupenaeus japonicus under the white spot syndrome virus challenge using RNA sequencing

Kaimin Hui; Qian Ren; Jun Cao

doi:10.1016/j.vetimm.2018.12.001

Insights into the intestine immune of Marsupenaeus japonicus under the white spot syndrome virus challenge using RNA sequencing

Vet Immunol Immunopathol. 2019 Feb:208:25-33. doi: 10.1016/j.vetimm.2018.12.001. Epub 2018 Dec 21.

Authors

Kaimin Hui¹, Qian Ren², Jun Cao³

Affiliations

¹ College of Marine Science and Engineering, Nanjing Normal University, Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, People's Republic of China.
² College of Marine Science and Engineering, Nanjing Normal University, Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, People's Republic of China; Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, Jiangsu, People's Republic of China. Electronic address: renqian0402@126.com.
³ College of Marine Science and Engineering, Nanjing Normal University, Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, People's Republic of China. Electronic address: cjinfor@163.com.

PMID: 30712789
DOI: 10.1016/j.vetimm.2018.12.001

Abstract

Intestine is not only the nutrients digestion and absorption centers, but also an important place of microbial infection. Therefore, intestine immunity plays a key defense means for the host against the invasion of pathogenic microorganisms. In this study, we use kuruma shrimp (Marsupenaeus japonicus) as a model to study the intestine immune characteristics of shrimp against WSSV through next-generation sequencing technique. A total of 63,458 and 44,350 unigenes were generated from the control sample and the WSSV infection sample, respectively. Based on homology searches, KEGG, GO, and COG analysis, 39,520 unigenes were annotated. Among them, 12,920 differentially expressed genes were identified. Some of them, including mucin, peritrophin, chitinase, et al., are involved in immune response. These results contribute to a better understanding of the intestine immune response of the shrimp to WSSV.

Keywords: Intestine immune; Marsupenaeus japonicus; Transcriptome; WSSV.

MeSH terms

Animals
Gene Expression Profiling
High-Throughput Nucleotide Sequencing
Host-Pathogen Interactions
Immunity, Innate / genetics
Intestines / immunology*
Penaeidae / anatomy & histology
Penaeidae / immunology*
Penaeidae / virology*
Phylogeny
Sequence Analysis, RNA
White spot syndrome virus 1*