Evaluation of a viral DNA-protein immunization strategy against African swine fever in domestic pigs

Vet Immunol Immunopathol. 2019 Feb;208:34-43. doi: 10.1016/j.vetimm.2018.11.018. Epub 2018 Dec 21.

Abstract

African swine fever virus (ASFV) causes serious disease in domestic pigs for which there is no vaccine currently available. ASFV is a large DNA virus that encodes for more than 150 proteins, thus making the identification of viral antigens that induce a protective immune response difficult. Based on the functional roles of several ASFV proteins found in previous studies, we selected combinations of ASFV recombinant proteins and pcDNAs-expressing ASFV genes, to analyze their ability to induce humoral and cellular immune responses in pigs. Pigs were immunized using a modified prime-boost approach with combinations of previously selected viral DNA and proteins, resulting in induction of antibodies and specific cell-mediated immune response, measured by IFN-γ ELISpots. The ability of antibodies from pigs immunized with various combinations of ASFV-specific antigens to neutralize infection in vitro, and antigen-specific activation of the cellular immune response were analyzed.

Keywords: African swine fever virus; Heterologous DNA-protein immunization; Immune response; Immunopathology; Plasmid-expressed antigen; Recombinant protein.

Publication types

  • Evaluation Study

MeSH terms

  • African Swine Fever / immunology
  • African Swine Fever / prevention & control*
  • African Swine Fever Virus
  • Animals
  • Antibodies, Viral / blood
  • Antigens, Viral / immunology
  • DNA, Viral / administration & dosage
  • DNA, Viral / immunology*
  • Enzyme-Linked Immunospot Assay
  • Immunity, Cellular
  • Interferon-gamma / immunology
  • Recombinant Proteins / administration & dosage
  • Recombinant Proteins / immunology
  • Sus scrofa
  • Swine
  • Viral Proteins / administration & dosage
  • Viral Proteins / immunology*
  • Viral Vaccines / administration & dosage
  • Viral Vaccines / immunology*

Substances

  • Antibodies, Viral
  • Antigens, Viral
  • DNA, Viral
  • Recombinant Proteins
  • Viral Proteins
  • Viral Vaccines
  • Interferon-gamma