CIPK11-Dependent Phosphorylation Modulates FIT Activity to Promote Arabidopsis Iron Acquisition in Response to Calcium Signaling

Dev Cell. 2019 Mar 11;48(5):726-740.e10. doi: 10.1016/j.devcel.2019.01.006. Epub 2019 Jan 31.

Abstract

Nutrient acquisition is entangled with growth and stress in sessile organisms. The bHLH transcription factor FIT is a key regulator of Arabidopsis iron (Fe) acquisition and post-translationally activated upon low Fe. We identified CBL-INTERACTING PROTEIN KINASE CIPK11 as a FIT interactor. Cytosolic Ca2+ concentration and CIPK11 expression are induced by Fe deficiency. cipk11 mutant plants display compromised root Fe mobilization and seed Fe content. Fe uptake is dependent on CBL1/CBL9. CIPK11 phosphorylates FIT at Ser272, and mutation of this target site modulates FIT nuclear accumulation, homo-dimerization, interaction with bHLH039, and transcriptional activity and affects the plant's Fe-uptake ability. We propose that Ca2+-triggered CBL1/9-mediated activation of CIPK11 and subsequent phosphorylation of FIT shifts inactive into active FIT, allowing regulatory protein interactions in the nucleus. This biochemical link between Fe deficiency and the cellular Ca2+ decoding machinery represents an environment-sensing mechanism to adjust nutrient uptake.

Keywords: CBL; CIPK11; FIT; bHLH039; iron deficiency; nucleocytoplasmic partitioning; protein interaction; protein mobility; protein phosphorylation; stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / metabolism*
  • Basic Helix-Loop-Helix Transcription Factors / metabolism*
  • Calcium Signaling / physiology*
  • Cell Nucleus / metabolism
  • Gene Expression Regulation, Plant*
  • Phosphorylation
  • Plant Roots / genetics
  • Plant Roots / metabolism*
  • Plants, Genetically Modified / metabolism
  • Protein-Serine-Threonine Kinases / metabolism*

Substances

  • Arabidopsis Proteins
  • Basic Helix-Loop-Helix Transcription Factors
  • FIT1 protein, Arabidopsis
  • At2g30360 protein, Arabidopsis
  • Protein-Serine-Threonine Kinases