Immunoaffinity isolation of a sialyl-Le(a) oligosaccharide from human milk

H Kitagawa; H Nakada; Y Numata; A Kurosaka; S Fukui; I Funakoshi; T Kawasaki; K Shimada; F Inagaki; I Yamashina

doi:10.1093/oxfordjournals.jbchem.a122516

Immunoaffinity isolation of a sialyl-Le(a) oligosaccharide from human milk

J Biochem. 1988 Oct;104(4):591-4. doi: 10.1093/oxfordjournals.jbchem.a122516.

Authors

H Kitagawa¹, H Nakada, Y Numata, A Kurosaka, S Fukui, I Funakoshi, T Kawasaki, K Shimada, F Inagaki, I Yamashina

Affiliation

¹ Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University.

PMID: 3071532
DOI: 10.1093/oxfordjournals.jbchem.a122516

Abstract

A cancer-associated antigen, sialyl-Le(a) oligosaccharide, was isolated from human milk using a monoclonal antibody recognizing carbohydrate moieties of mucin-type glycoproteins. The structure was identified as: (Formula: see text) based on 500-MHz 1H-NMR spectroscopy. This oligosaccharide comprises 0.07% of sialyloligosaccharides in human milk. The NMR spectra of two fellow oligosaccharides, Le(a) oligosaccharide (or lacto-N-fucopentaose II) and LS-tetrasaccharide a, are also given.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Monoclonal / immunology
Antigens, Tumor-Associated, Carbohydrate / isolation & purification*
Chromatography, Affinity / methods
Chromatography, High Pressure Liquid
Humans
Immunosorbent Techniques
Magnetic Resonance Spectroscopy
Milk, Human / metabolism*
Mucins / biosynthesis
Oligosaccharides / isolation & purification*

Substances

Antibodies, Monoclonal
Antigens, Tumor-Associated, Carbohydrate
Mucins
Oligosaccharides
sialyl-Le(a) oligosaccharide