Cellular antioxidant activity and in vitro inhibition of α-glucosidase, α-amylase and pancreatic lipase of oregano polyphenols under simulated gastrointestinal digestion

Food Res Int. 2019 Feb;116:676-686. doi: 10.1016/j.foodres.2018.08.096. Epub 2018 Aug 31.


Different oregano species have been traditionally used as infusions in folk medicine. Oregano medicinal properties, such as antioxidant and anti-inflammatory, have been partially attributed to its polyphenolic content. However, information regarding bioaccessibility of oregano polyphenols is limited. Cell-based antioxidant activity, and in vitro hypoglycemic, and hypolipidemic properties of polyphenolic extracts from three species of oregano species, namely, Hedeoma patens (HP), Lippia graveolens (LG) and Lippia palmeri (LP), subjected to simulated gastrointestinal digestion were evaluated. LC-TOF-MS analysis of HP, LG and LP allowed the identification of 9 flavonoids and 6 hydroxycinnamic acid derivatives with nutraceutical significance. Oregano polyphenolic extracts and digests from HP, LG, and LP exhibited cellular antioxidant capacity, hypoglycemic and hypolipidemic properties. Altogether, our results suggest that HP, LG and LP polyphenols exhibit potential for use as hypoglycemic, hypolipidemic, and antioxidant agents.

Keywords: Bioavailability; Gastrointestinal digestion; Hedeoma patens; Lippia graveolens; Lippia palmeri; Polyphenols.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / isolation & purification
  • Antioxidants / pharmacology*
  • Caco-2 Cells
  • Chromatography, High Pressure Liquid
  • Digestion*
  • Gastric Acid / chemistry
  • Glycoside Hydrolase Inhibitors / isolation & purification
  • Glycoside Hydrolase Inhibitors / pharmacology*
  • Humans
  • Hypolipidemic Agents / isolation & purification
  • Hypolipidemic Agents / pharmacology*
  • Intestinal Secretions / chemistry
  • Lipase / antagonists & inhibitors*
  • Lipase / metabolism
  • Origanum / chemistry*
  • Oxidation-Reduction
  • Oxidative Stress / drug effects
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Polyphenols / isolation & purification
  • Polyphenols / pharmacology*
  • Spectrometry, Mass, Electrospray Ionization


  • Antioxidants
  • Glycoside Hydrolase Inhibitors
  • Hypolipidemic Agents
  • Plant Extracts
  • Polyphenols
  • Lipase