Resistance gene cloning from a wild crop relative by sequence capture and association genetics

Nat Biotechnol. 2019 Feb;37(2):139-143. doi: 10.1038/s41587-018-0007-9. Epub 2019 Feb 4.


Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Mapping
  • Cloning, Molecular*
  • Crops, Agricultural / genetics*
  • Disease Resistance / genetics*
  • Genes, Plant*
  • Genetic Association Studies
  • Genetic Variation
  • Genomics
  • Genotype
  • Models, Genetic
  • Phenotype
  • Phylogeny
  • Plant Diseases / genetics*
  • Polymorphism, Single Nucleotide
  • Seedlings
  • Triticum / genetics