MiR-374b reduces cell proliferation and cell invasion of cervical cancer through regulating FOXM1

Eur Rev Med Pharmacol Sci. 2019 Jan;23(2):513-521. doi: 10.26355/eurrev_201901_16863.

Abstract

Objective: Deregulation of microRNAs (miRNAs) has been identified as critical event in tumor initiation and progression. We aimed to explore the role of miR-374b in cervical cancer progression.

Patients and methods: MiR-374b expression was detected using qRT-PCR in cervical cancer tissues compared with normal counterparts. Cell proliferation and invasion ability were detected using Cell Counting Kit-8 (CCK8) cell proliferation and transwell invasion assay. Dual luciferase reporter assay, qRT-PCR, and Western blot analysis were used to demonstrate that FOXM1 was a target of miR-374b.

Results: We demonstrated that downregulation of miR-374b was frequently examined in cervical cancer tissues compared with normal counterparts. Furthermore, we showed the lower miR-374b expression associated with lymph node metastasis and advanced FIGO stage in patients with cervical cancer. Furthermore, ectopic expression of miR-374b could significantly decrease cell proliferation and invasion ability. However, inhibition of miR-374b had opposite effects. Dual luciferase reporter assay, qRT-PCR, and Western blot analysis revealed that miR-374b overexpression suppressed cell proliferation and invasion ability via affecting FOXM1 expression.

Conclusions: These results indicated that miR-374b acted as tumor suppressor and may serve as a potential target for cervical cancer treatment.

MeSH terms

  • Cell Proliferation
  • Female
  • Forkhead Box Protein M1 / genetics
  • Forkhead Box Protein M1 / metabolism*
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Middle Aged
  • Tumor Cells, Cultured
  • Uterine Cervical Neoplasms / metabolism*
  • Uterine Cervical Neoplasms / pathology

Substances

  • FOXM1 protein, human
  • Forkhead Box Protein M1
  • MIRN374 microRNA 374, human
  • MicroRNAs