FGF2-FGFR1 signaling regulates release of Leukemia-Protective exosomes from bone marrow stromal cells

Elife. 2019 Feb 5;8:e40033. doi: 10.7554/eLife.40033.

Abstract

Protective signaling from the leukemia microenvironment leads to leukemia cell persistence, development of resistance, and disease relapse. Here, we demonstrate that fibroblast growth factor 2 (FGF2) from bone marrow stromal cells is secreted in exosomes, which are subsequently endocytosed by leukemia cells, and protect leukemia cells from tyrosine kinase inhibitors (TKIs). Expression of FGF2 and its receptor, FGFR1, are both increased in a subset of stromal cell lines and primary AML stroma; and increased FGF2/FGFR1 signaling is associated with increased exosome secretion. FGFR inhibition (or gene silencing) interrupts stromal autocrine growth and significantly decreases secretion of FGF2-containing exosomes, resulting in less stromal protection of leukemia cells. Likewise, Fgf2 -/- mice transplanted with retroviral BCR-ABL leukemia survive significantly longer than their +/+ counterparts when treated with TKI. Thus, inhibition of FGFR can modulate stromal function, reduce exosome secretion, and may be a therapeutic option to overcome resistance to TKIs.

Editorial note: This article has been through an editorial process in which the authors decide how to respond to the issues raised during peer review. The Reviewing Editor's assessment is that all the issues have been addressed (see decision letter).

Keywords: FGF2; FGFR1; bone marrow stroma; cancer biology; drug resistance; exosomes; human; human biology; medicine; microenvironment; mouse.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Survival
  • Cells, Cultured
  • Disease Models, Animal
  • Exosomes / metabolism*
  • Fibroblast Growth Factor 2 / metabolism*
  • Humans
  • Leukemia, Myeloid, Acute / pathology*
  • Mesenchymal Stem Cells / metabolism*
  • Mice
  • Mice, Knockout
  • Receptor, Fibroblast Growth Factor, Type 1 / metabolism*
  • Signal Transduction*

Substances

  • Fibroblast Growth Factor 2
  • Receptor, Fibroblast Growth Factor, Type 1