Spectroscopic analysis reveals the effect of a single nucleotide bulge on G-quadruplex structures

Chem Commun (Camb). 2019 Feb 26;55(18):2616-2619. doi: 10.1039/c8cc09929d.


Here we investigate and reveal the effect of bulge position and bulge identity on G-quadruplexes using label-free spectroscopic techniques. Notably, we report significant differences in the spectroscopic features of bulged DNA and RNA G-quadruplexes, and demonstrate that intrinsic fluorescence can be generally used to detect the formation of canonical and non-canonical G-quadruplexes.

MeSH terms

  • Base Sequence
  • Circular Dichroism
  • DNA / chemistry*
  • G-Quadruplexes*
  • Nucleic Acid Conformation
  • Oligonucleotides / chemistry
  • Phase Transition
  • Spectrometry, Fluorescence
  • Ultraviolet Rays


  • Oligonucleotides
  • DNA