The local shear wave speed (in meters per second) and Young modulus (in kilopascals) of normal thyroid glands were defined in healthy children to determine reference values.This prospective study assessed 107 pediatric cases (age interval, 3-17 years; mean, 9.37 ± 3.68 years), including 52 boys and 55 girls. The study group included children without thyroid gland disease (autoimmune, infectious, or neoplastic). Data about the sex, age, weight, height, and body mass index (BMI) of the subjects were recorded for all subjects. Elasticity values were measured from 3 different sites in both thyroid lobes and averaged.Median values for elasticity and shear wave velocity measured in bilateral thyroid lobes were 6.38 ± 1.97 kPa (range, 3.00-12.5 kPa) and 1.45 ± 0.21 m/s (range, 1.03-2.04 m/s) on the right and 8.81 ± 3.00 kPa (range, 3.80-22.6 kPa) and 1.69 ± 0.26 m/s (range, 1.13-2.68 m/s) on the left. There was no significant difference between the elasticity values for the right and left thyroid lobes between boys and girls. There was a positive correlation between right thyroid lobe mean elasticity (in kilopascals) and shear wave velocity (in meters per second) values with age, BMI, and right thyroid lobe volume in the whole group. No significant correlation was found between left thyroid lobe mean elasticity (in kilopascals) and shear wave velocity (in meters per second) values with age, BMI, and left thyroid lobe volume in the study population.This study determined mean elasticity and shear wave velocity values for thyroid gland in healthy children. This information can be used as a baseline for the investigation of thyroid diseases.