Nucleotide sequence of a DNA fragment that contains the abi gene of the ColIb plasmid

Plasmid. 1988 Nov;20(3):194-206. doi: 10.1016/0147-619x(88)90026-1.

Abstract

A 1989-bp PstI DNA fragment from the ColIb plasmid, which contains the abi gene that is necessary for the abortive response to infections by bacteriophage BF23 or T5, was sequenced. A candidate open reading frame for the abi gene has been suggested on the basis of a Shine-Dalgarno sequence appropriately placed ahead of its ATG initiation codon, a promoter upstream from the Shine-Dalgarno sequence, and a location compatible with deletion mapping. The polypeptide that would be coded by this open reading frame is 89 amino acids long and strongly hydrophobic. A promoter that could serve this open reading frame was detected by exonuclease III "footprinting" using RNA polymerase from uninfected Escherichia coli as the DNA-binding protein.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Coliphages / genetics
  • Coliphages / physiology*
  • DNA, Bacterial / genetics
  • Escherichia coli / genetics*
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Plasmids*
  • Restriction Mapping

Substances

  • DNA, Bacterial

Associated data

  • GENBANK/J03314