Dynamic Thiol/Disulphide Homeostasis in Children With Nephrotic Syndrome

Nephron. 2019;142(1):17-25. doi: 10.1159/000496619. Epub 2019 Feb 6.

Abstract

Background: There is a growing body of evidence showing that there is an association between oxidative damage and kidney injury in patients with nephrotic syndrome (NS). Dynamic thiol/disulphide homeostasis (DTDH) is an important antioxidant system. The aim of this study was to determine if DTDH could be used as a biomarker of antioxidant status in pediatric NS patients using a novel automated method.

Methods: The study included 39 patients with NS and 40 healthy controls. The DTDH level was measured using a calorimetric and automated method developed by Erel and Neselioglu. In the NS group, DTDH was measured during first-attack episode, relapse, and remission.

Results: The native thiol, total thiol, and disulphide levels were significantly lower, the native thiol/total thiol ratio was significantly lower, and the disulphide/native thiol and disulphide/total thiol ratios were significantly higher in the NS patients during relapse and first-attack episode than during remission and in the controls. In addition, the native thiol and total thiol levels, and the native thiol/total thiol ratio were significantly lower, and the disulphide/native thiol and disulphide/total thiol ratios were significantly higher in the NS group during remission than those in the control group.

Conclusions: The present findings show that DTDH shifted toward disulphide formation in the NS patients, even during remission. A clearer understanding of the mechanism of this disruption to homeostasis in NS patients might lead to the development of novel therapeutic strategies.

Keywords: Anti-oxidants; Childhood; Nephrotic syndrome; Oxidative stress; Thiol/disulphide homeostasis.

MeSH terms

  • Adolescent
  • Case-Control Studies
  • Child
  • Child, Preschool
  • Disulfides / metabolism*
  • Female
  • Homeostasis*
  • Humans
  • Male
  • Nephrotic Syndrome / metabolism*
  • Prospective Studies
  • Sulfhydryl Compounds / metabolism*

Substances

  • Disulfides
  • Sulfhydryl Compounds