Firefly luciferase as a tool in molecular and cell biology

Anal Biochem. 1988 Nov 15;175(1):5-13. doi: 10.1016/0003-2697(88)90353-3.


The unique properties of firefly luciferase and the cloning of the gene for this enzyme have spawned a number of novel applications of this protein. We summarize a few of these applications including its use as a reporter gene, as a model for the study of protein import into peroxisomes, and as a component of a heterologous gene expression system.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Cloning, Molecular
  • Coleoptera / enzymology
  • Luciferases* / genetics
  • Microbodies / metabolism
  • Models, Biological
  • Mutation
  • Proteins / metabolism


  • Proteins
  • Luciferases