Murine Models of Bone Sarcomas

Methods Mol Biol. 2019;1914:331-342. doi: 10.1007/978-1-4939-8997-3_18.

Abstract

This chapter describes the procedures for inducing bone sarcoma in mice. Two models based on inoculation of cancer cells in paraosseous and intraosseous site will be described. In addition to providing technical aspects of anesthesia and surgical options, key information of cell preparation and postoperative follow-up will be discussed.

Keywords: Bone sarcoma; Cell-injection; Chondrosarcoma; Ewing sarcoma; Genetically-engineered mouse models; Murine preclinical models; Osteosarcoma.

MeSH terms

  • Animals
  • Bone Neoplasms / diagnostic imaging
  • Bone Neoplasms / genetics
  • Bone Neoplasms / pathology*
  • Bone and Bones / diagnostic imaging
  • Bone and Bones / pathology
  • Cell Culture Techniques / instrumentation
  • Cell Culture Techniques / methods
  • Cell Line, Tumor / transplantation
  • Humans
  • Mice
  • Mice, Inbred NOD
  • Mice, Knockout
  • Mice, Nude
  • Mice, SCID
  • Osteosarcoma / diagnostic imaging
  • Osteosarcoma / genetics
  • Osteosarcoma / pathology*
  • Rats
  • Rats, Sprague-Dawley
  • Sarcoma, Experimental / diagnostic imaging
  • Sarcoma, Experimental / genetics
  • Sarcoma, Experimental / pathology*
  • X-Ray Microtomography
  • Xenograft Model Antitumor Assays / instrumentation
  • Xenograft Model Antitumor Assays / methods*