The genetic requirements of fatty acid import by Mycobacterium tuberculosis within macrophages

Elife. 2019 Feb 8:8:e43621. doi: 10.7554/eLife.43621.


Mycobacterium tuberculosis (Mtb) imports and metabolizes fatty acids to maintain infection within human macrophages. Although this is a well-established paradigm, the bacterial factors required for fatty acid import are poorly understood. Previously, we found that LucA and Mce1 are required for fatty acid import in Mtb (Nazarova et al., 2017). Here, we identified additional Mtb mutants that have a reduced ability to import a fluorescent fatty acid substrate during infection within macrophages. This screen identified the novel genes as rv2799 and rv0966c as be necessary for fatty acid import and confirmed the central role for Rv3723/LucA and putative components of the Mce1 fatty acid transporter (Rv0200/OmamB, Rv0172/Mce1D, and Rv0655/MceG) in this process.

Keywords: LucA; Mce1; Mycobacterium tuberculosis; fatty acids; infectious disease; macrophages; microbiology; tuberculosis.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Bacterial Proteins / genetics*
  • Fatty Acids / genetics*
  • Fatty Acids / metabolism
  • Host-Pathogen Interactions / genetics
  • Humans
  • Macrophages / metabolism
  • Macrophages / microbiology
  • Mutant Proteins / genetics
  • Mycobacterium tuberculosis / genetics*
  • Mycobacterium tuberculosis / metabolism
  • Mycobacterium tuberculosis / pathogenicity
  • Tuberculosis / genetics*
  • Tuberculosis / microbiology


  • Bacterial Proteins
  • Fatty Acids
  • Mutant Proteins
  • mammalian cell entry protein Mce1A, Mycobacterium tuberculosis