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. 1998 Feb;17(4):262-267.
doi: 10.1007/s002990050389.

Microprojectile Bombardment-Mediated Transformation of Lilium Longiflorum

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Microprojectile Bombardment-Mediated Transformation of Lilium Longiflorum

A A Watad et al. Plant Cell Rep. .

Abstract

We have obtained transgenic lily (Lilium longiflorum) plants after microprojectile bombardment, using the Biolistics PDS 1000/He system, of morphogenic calli derived from bulblet scales, followed by bialaphos selection. Parameters which gave the highest transient uidA expression were used: a bombardment pressure of 1100 psi, a target distance of 6 cm and a 48-h preculture on medium with 3% sucrose. A total of 1800 morphogenic calli were co-bombarded with plasmids containing either the uidA reporter or PAT selectable marker genes. After bombardment, the calli were exposed to 2 mg/l bialaphos. Only 72 of the shoot-forming calli (4%) survived. The 72 shoot clusters produced 342 shoots on elongation medium containing 0.5 mg/l bialaphos. Only 55 plantlets survived subsequent exposure to 2.0 mg/l bialaphos. PCR analysis indicated that 19 of these plantlets contained the PAT transgene. Southern analysis of 3 of the plants indicated that all contained the PAT gene.

Keywords: Adventitious shoot regeneration; Bialaphos; Key words Lily; Transformation.

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