Baicalin induced colon cancer cells apoptosis through miR-217/DKK1-mediated inhibition of Wnt signaling pathway

Mol Biol Rep. 2019 Apr;46(2):1693-1700. doi: 10.1007/s11033-019-04618-9. Epub 2019 Feb 8.


To analyze the anti-tumor mechanism of Baicalin in human colon cancer. The MTT assay and colony formation assay demonstrated that Baicalin treatment inhibits the proliferation of DLD1 and HCT-116 cells. The apoptosis rates were induced upon Baicalin treatment and which was determined by FACS. The qPCR and western blot analysis showed that Baicalin promotes expression of DKK1 (Dickkopf), an important antagonist of Wnt signaling pathway, thereby reduces the expression of its downstream protein β-catenin and c-Myc. Reduction of DKK1 expression by siRNA attenuates β-catenin and c-Myc expression. microRNA-217 (miR-217) is decreased upon Baicalin treatment. Moreover, DKK1 is identified as the direct downstream target gene of miR-217 through the dual-luciferase reporter assay. miR-217/DKK1-mediated inhibition of Wnt signaling pathway participate in apoptosis induced by Baicalin.

Keywords: Apoptosis; Baicalin; DKK1; MiR-217; Wnt signaling pathway.

MeSH terms

  • Apoptosis / drug effects
  • Apoptosis / genetics
  • Cell Line, Tumor / drug effects
  • Cell Proliferation / drug effects
  • Colon / metabolism
  • Colonic Neoplasms / drug therapy
  • Colonic Neoplasms / genetics*
  • Colonic Neoplasms / metabolism
  • Flavonoids / metabolism
  • Flavonoids / pharmacology*
  • HCT116 Cells
  • Humans
  • Intercellular Signaling Peptides and Proteins / genetics
  • Intercellular Signaling Peptides and Proteins / metabolism*
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Wnt Signaling Pathway / drug effects*
  • Wnt Signaling Pathway / genetics


  • DKK1 protein, human
  • Flavonoids
  • Intercellular Signaling Peptides and Proteins
  • MIRN217 microRNA, human
  • MicroRNAs
  • baicalin