Chemical screening of novel strigolactone agonists that specifically interact with DWARF14 protein

Bioorg Med Chem Lett. 2019 Apr 1;29(7):938-942. doi: 10.1016/j.bmcl.2019.01.010. Epub 2019 Jan 11.

Abstract

Strigolactones (SLs) are a class of plant hormones that regulate shoot branching as well as being known as root-derived signals for parasitic and symbiotic interactions. The physical interaction between SLs and the DWARF14 (D14) receptor family can be examined by differential scanning fluorimetry (DSF) that monitors the changes in protein melting temperature (Tm). The Tm of D14 is lowered by bioactive SLs in DSF analysis. In this report, we screened the compounds that lower the Tm of Arabidopsis D14 (AtD14) as potential candidates for SL agonists using DSF analysis. Subsequent physiological analyzes revealed that 113D10 acts as a novel SL agonist in a D14-dependent manner. Intriguingly, 113D10 has a chemical structure different from natural SLs in that it does not possess an enol ether bond that connects to a methylbutenolide moiety. Moreover, 113D10 does not stimulate seed germination of root parasitic plants. Accordingly, 113D10 can be a useful tool for SL studies and agricultural applications.

Keywords: Agonist; Chemical screening; Receptor; Strigolactone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Dose-Response Relationship, Drug
  • Gene Expression Regulation, Plant / drug effects*
  • Lactones / administration & dosage
  • Lactones / chemistry
  • Lactones / pharmacology*
  • Molecular Structure
  • Mutation
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / metabolism*
  • Structure-Activity Relationship

Substances

  • Arabidopsis Proteins
  • DWARF14 protein, Arabidopsis
  • Lactones
  • Receptors, Cell Surface