An improved plasmid size standard, 39R861

Robert A Moran; Sashindran Anantham; Ruth M Hall

doi:10.1016/j.plasmid.2019.01.002

An improved plasmid size standard, 39R861

Plasmid. 2019 Mar:102:6-9. doi: 10.1016/j.plasmid.2019.01.002. Epub 2019 Feb 7.

Authors

Robert A Moran¹, Sashindran Anantham², Ruth M Hall²

Affiliations

¹ School of Life and Environmental Sciences, The University of Sydney, NSW, Australia. Electronic address: robert.moran@sydney.edu.au.
² School of Life and Environmental Sciences, The University of Sydney, NSW, Australia.

PMID: 30738800
DOI: 10.1016/j.plasmid.2019.01.002

Abstract

The four plasmids in E. coli 39R861 have been used as size standards for closed-circular plasmid DNA since the 1980s. However, their sizes had only been estimated. Here, the complete sequence of the 61.3 kb cryptic FII plasmid p39R861-3 is reported, and the sizes of p39R861-4, pSa and NTP168 were determined to be 155.8, 40.1 and 6.8 kb, respectively. An improved size standard, 39R861+, was created by adding two sequenced, compatible plasmids to 39R861 via conjugation. The 94.8 kb conjugative B/O plasmid p838B-R mobilised the cryptic, 2.0 kb pBuzz. The six plasmids in 39R861+ are all stably maintained.

Keywords: 39R861; Electrophoresis; Plasmid sizing; Standard.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
DNA, Bacterial / genetics
Plasmids / genetics*

Substances

DNA, Bacterial