Orally administered collagen peptide protects against UVB-induced skin aging through the absorption of dipeptide forms, Gly-Pro and Pro-Hyp

Biosci Biotechnol Biochem. 2019 Jun;83(6):1146-1156. doi: 10.1080/09168451.2019.1580559. Epub 2019 Feb 11.


Collagen hydrolysate is a well-known nutritional supplement for the improvement of healthy skin. Here, collagen peptide NS (CPNS) from fish scale was prepared, and its physicochemical properties were investigated. Gly-Pro was revealed as a representative low molecular weight peptide of CPNS, by performing prep-HPLC and LC-MS/MS. CPNS treatment attenuated matrix metalloproteinase-1 production and increased the synthesis of type 1 procollagen in HDF cells. After orally administering CPNS to rats, the plasma concentrations of Gly-Pro and Pro-Hyp increased dramatically. To examine the protective effects of CPNS against ultraviolet B (UVB)-induced photoaging in vivo, the dorsal skins of hairless mice were exposed to UVB and supplemented with CPNS for 12 weeks. The CPNS consumption significantly attenuated UVB-induced wrinkle formation, transepidermal water loss, and epidermis thickness, and increased skin hydration. Collectively, these results suggest that bioactive peptides of CPNS, Gly-Pro and Pro-Hyp, exert beneficial effects on skin health.

Keywords: Collagen hydrolysate; Gly-Pro; Pro-Hyp; bioactive peptide; skin health.

MeSH terms

  • Administration, Oral
  • Animals
  • Cells, Cultured
  • Chromatography, High Pressure Liquid / methods
  • Collagen Type I / blood
  • Collagen Type I / chemistry*
  • Dipeptides / administration & dosage
  • Dipeptides / blood
  • Dipeptides / chemistry
  • Dipeptides / pharmacology*
  • Female
  • Humans
  • Hydroxyproline / chemistry*
  • Matrix Metalloproteinase 1 / metabolism
  • Mice
  • Mice, Hairless
  • Molecular Weight
  • Proline / chemistry*
  • Rats
  • Rats, Sprague-Dawley
  • Skin Aging / drug effects*
  • Skin Aging / radiation effects*
  • Tandem Mass Spectrometry / methods
  • Ultraviolet Rays*


  • Collagen Type I
  • Dipeptides
  • glycylproline
  • Proline
  • MMP1 protein, human
  • Matrix Metalloproteinase 1
  • Hydroxyproline