Protocol to Generate Ureteric Bud Structures from Human iPS Cells

Shin-Ichi Mae; Makoto Ryosaka; Kenji Osafune

doi:10.1007/978-1-4939-9021-4_10

Protocol to Generate Ureteric Bud Structures from Human iPS Cells

Methods Mol Biol. 2019:1926:117-123. doi: 10.1007/978-1-4939-9021-4_10.

Authors

Shin-Ichi Mae¹, Makoto Ryosaka¹, Kenji Osafune²

Affiliations

¹ Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan.
² Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan. osafu@cira.kyoto-u.ac.jp.

PMID: 30742267
DOI: 10.1007/978-1-4939-9021-4_10

Abstract

The generation of ureteric bud (UB), which is the renal progenitor that gives rise to renal collecting ducts and lower urinary tract, from human-induced pluripotent stem cells (hiPSCs) provides a cell source for studying the development of UB and kidney disease. Here we describe a stepwise and efficient two-dimensional differentiation method of hiPSCs into Wolffian duct (WD) cells. We also describe how to generate three-dimensional WD epithelial structures that can differentiate into UB-like structures.

Keywords: Anterior intermediate mesoderm; Anterior primitive streak; Human pluripotent stem cells; Ureteric bud; Wolffian duct.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation / physiology
Embryo, Mammalian / cytology*
Gene Expression Regulation, Developmental
Humans
Induced Pluripotent Stem Cells / cytology*
Kidney / cytology*
Mesoderm / cytology
Ureter / cytology*