Gene Editing in 3D Cultured Nephron Progenitor Cell Lines

Methods Mol Biol. 2019;1926:151-159. doi: 10.1007/978-1-4939-9021-4_13.

Abstract

During kidney development, Six2+ nephron progenitor cells (NPCs) generate nephrons, the functional units of the kidney. Isolating and expanding NPCs in vitro have enabled wide applications in both basic and translational research. Here we describe the methods to derive NPC lines from mouse embryonic kidneys in a 3D culture format as well as gene editing in the NPC lines.

Keywords: 3D culture; CRISPR-Cas9; Gene editing; NPSR culture medium; Nephron progenitor cell (NPC).

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cells, Cultured
  • Clustered Regularly Interspaced Short Palindromic Repeats / genetics
  • Gene Editing / methods
  • Mice
  • Nephrons / cytology*
  • Stem Cells / cytology*