Genetic transformation of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' via microprojectile bombardment

D K Becker; B Dugdale; M K Smith; R M Harding; J L Dale

doi:10.1007/s002990050004

Genetic transformation of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' via microprojectile bombardment

Plant Cell Rep. 2000 Jan;19(3):229-234. doi: 10.1007/s002990050004.

Authors

D K Becker¹, B Dugdale¹, M K Smith², R M Harding¹, J L Dale¹

Affiliations

¹ Centre for Molecular Biotechnology, School of Life Sciences, Queensland University of Technology, GPO Box 2434, Brisbane, Queensland 4001, Australia e-mail: j.dale@qut.edu.au Fax: (+61) 7 3864 1534, , , , , , AU.
² Maroochy Research Station, Queensland Horticulture Institute, Department of Primary Industries, Queensland, Australia, , , , , , AU.

PMID: 30754900
DOI: 10.1007/s002990050004

Abstract

An effective method has been developed for the stable transformation and regeneration of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' by microprojectile bombardment. Embryogenic cell suspensions were initiated using immature male flowers as the explant. Cells were co-bombarded with the neomycin phosphotransferase (nptII) selectable marker gene under the control of a banana bunchy top virus (BBTV) promoter or the CaMV 35S promoter, and either the β-glucuronidase (uidA) reporter gene or BBTV genes under the control of the maize polyubiquitin promoter. Plants were regenerated, under selection with kanamycin, that were co-transformed with nptII and either the uidA or BBTV genes. Molecular characterisation of transformants demonstrated that the transgenes had been stably integrated into the banana genome.

Keywords: Banana; Key words Musa; Microprojectile bombardment; Transformation.