PILS6 is a temperature-sensitive regulator of nuclear auxin input and organ growth in Arabidopsis thaliana

Proc Natl Acad Sci U S A. 2019 Feb 26;116(9):3893-3898. doi: 10.1073/pnas.1814015116. Epub 2019 Feb 12.


Temperature modulates growth and development throughout the entire lifecycle of a plant. High temperature (HT) triggers the auxin biosynthesis-dependent growth in aerial tissues. On the other hand, the contribution of auxin to HT-induced root growth is currently under debate. Here we show that the putative intracellular auxin carrier PIN-LIKES 6 (PILS6) is a negative regulator of organ growth and that its abundance is highly sensitive to HT. PILS6 localizes to the endoplasmic reticulum and limits the nuclear availability of auxin, consequently reducing the auxin signaling output. HT represses the PILS6 protein abundance, which impacts on PILS6-dependent auxin signaling in roots and root expansion. Accordingly, we hypothesize that PILS6 is part of an alternative mechanism linking HT to auxin responses in roots.

Keywords: PILS proteins; auxin signaling; endoplasmic reticulum; high temperature; root growth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics
  • Arabidopsis / growth & development*
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Biological Transport
  • Cell Nucleus / genetics
  • Cell Nucleus / metabolism
  • Endoplasmic Reticulum / genetics
  • Endoplasmic Reticulum / metabolism
  • Gene Expression Regulation, Plant
  • Hot Temperature
  • Indoleacetic Acids / metabolism*
  • Membrane Transport Proteins / genetics
  • Membrane Transport Proteins / metabolism*
  • Plant Growth Regulators / genetics*
  • Plant Growth Regulators / metabolism
  • Plant Roots / genetics*
  • Plant Roots / growth & development
  • Plant Roots / metabolism
  • Signal Transduction
  • Temperature


  • AT5G01990.1 protein, Arabidopsis
  • Arabidopsis Proteins
  • Indoleacetic Acids
  • Membrane Transport Proteins
  • Plant Growth Regulators