In the mammalian eye the tissue layers which effectively separate the neural retina and the transparent refractive media from the circulating blood are generally known as the blood-retinal barrier (BRB) and blood aqueous barrier (BAB) respectively. These have been delineated by morphologically identifying the ultrastructural features which impede the passage of molecules from the blood to the working parts of the eye; physiologically, there is a certain amount of overlap in their function, as there is free diffusion of solutes between the aqueous humour, the vitreous humour and the interstitial tissue of the neural retina. Notwithstanding, it is convenient and appropriate to maintain the distinction as the more recent work has focused on the fine structural aspects of these barrier systems. The abundant literature which describes the work leading to the identification and characterisation of these barriers has been reviewed in depth with great authority by several eminent researchers, notably the late Giuseppina Raviola, and Jose Cunha-Vaz. Certain pathological conditions, trauma--including surgical manipulation, and chemical irritation of the eye can cause significant disruption of these barriers with important clinical consequences. In our laboratory interest has centred around vascular and epithelial changes which occur when the eye is injured and which can lead to a breakdown of the BAB. More recently attention has been drawn to functional and behavioural differences between species, particularly with regard to the relative stability of the barrier.