Overexpression of StDREB2 Transcription Factor Enhances Drought Stress Tolerance in Cotton ( Gossypium barbadense L.)

Genes (Basel). 2019 Feb 14;10(2):142. doi: 10.3390/genes10020142.

Abstract

Drought stress significantly restricts plant growth and crop productivity. Cotton is the most important textile fiber and oilseed crop worldwide, and its cultivation is affected by drought stress, particularly in dry regions. Improving cotton tolerance to drought stress using the advanced genetic engineering technologies is a promising strategy to maintain crop production and fiber quality and meet the increasing worldwide fiber and oil demand. Dehydration-responsive element binding (DREB) transcription factors play a main role in regulating stresses-tolerance pathways in plant. This study investigated whether potato DREB2 (StDREB2) overexpression can improve drought tolerance in cotton. StDREB2 transcription factor was isolated and overexpressed in cotton. Plant biomass, boll number, relative water content, soluble sugars content, soluble protein content, chlorophyll content, proline content, gas-exchange parameters, and antioxidants enzymes (POD, CAT, SOD, GST) activity of the StDREB2-overexpressing cotton plants were higher than those of wild type plants. By contrast, the contents of malondialdehyde, hydrogen peroxide and superoxide anion of StDREB2-overexpressing transgenic plants were significantly lower than that of the wild type plants. Moreover, the transgenic cotton lines revealed higher expression levels of antioxidant genes (SOD, CAT, POD, GST) and stress-tolerant genes (GhERF2, GhNAC3, GhRD22, GhDREB1A, GhDREB1B, GhDREB1C) compared to wild-type plants. Taken together, these findings showed that StDREB2 overexpression augments drought stress tolerance in cotton by inducing plant biomass, gas-exchange characteristics, reactive oxygen species (ROS) scavenging, antioxidant enzymes activities, osmolytes accumulation, and expression of stress-related genes. As a result, StDREB2 could be an important candidate gene for drought-tolerant cotton breeding.

Keywords: StDREB2; antioxidants; drought; genes expression; osmolytes; transgenic cotton.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis Proteins / genetics
  • Chlorophyll / genetics
  • Droughts*
  • Gene Expression Regulation, Plant / genetics
  • Gossypium / genetics*
  • Gossypium / growth & development
  • Plant Proteins / genetics
  • Plant Roots / genetics
  • Plant Roots / growth & development
  • Plants, Genetically Modified / genetics
  • Reactive Oxygen Species / metabolism
  • Salt Tolerance / genetics
  • Solanum tuberosum / genetics
  • Stress, Physiological / genetics*
  • Transcription Factors / genetics*

Substances

  • Arabidopsis Proteins
  • DREB2A protein, Arabidopsis
  • Plant Proteins
  • Reactive Oxygen Species
  • Transcription Factors
  • Chlorophyll