Circulating CD14 + CD163 + CD209 + M2-like monocytes are associated with the severity of infection in Helicobacter pylori-positive patients

Mol Immunol. 2019 Apr;108:13-22. doi: 10.1016/j.molimm.2019.01.017. Epub 2019 Feb 13.

Abstract

Helicobacter pylori (H. pylori) initiates a robust host immune response and subsequently results in chronic inflammation in the gastric mucosa. This study monitored the circulating monocyte subsets and measured the plasma levels of IL-10 and IL-12 in response to H. pylori infection in 35 H. pylori-associated gastritis patients and 14 healthy controls. We found that the numbers of CD14+CD163-CD64+ M1-like monocytes as well as CD14+CD163+CD206+, CD14+CD163+CD209+, and CD14+CD163+IL-10+ M2-like monocytes were significantly increased in H. pylori-infected patients in comparison with the controls, accompanied by higher levels of plasma IL-10. In addition, IL-10 production was significantly higher in the stimulated M2-like cells from patients with H. pylori infection compared with controls. Moreover, the H. pylori-infected patients with CagA- or VacA-positive strains had a significantly higher number of CD14+CD163+CD206+, CD14+CD163+CD209+, CD14+CD163+IL-10+ monocytes compared to those with CagA- or VacA-negative strains. Furthermore, patients suffering from H. pylori-positive peptic ulcers had a greater number of CD14+CD163+CD209+ monocytes than H. pylori-positive nonatrophic or atrophic gastritis patients, with the numbers of CD14+CD163+CD209+ monocytes positively correlated with the extent of H. pylori infection. Notably, the triple anti-H. pylori therapy significantly reduced the numbers of CD14+CD163+CD206+ and CD14+CD163+CD209+ monocyte subsets. In conclusion, CD14+CD163+CD209+ M2-like monocyte subsets are increased in H. pylori infection, especially in patients with peptic ulcers. CD14+CD163+CD209+ M2-like monocytes are positively associated with the severity of H. pylori infection.

Keywords: Gastritis; Helicobacter pylori infection; IL-10; Monocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Antigens, CD / metabolism*
  • Bacterial Proteins / metabolism
  • Cell Differentiation
  • Cell Movement*
  • Cytokines / blood
  • Female
  • Helicobacter Infections / blood*
  • Helicobacter Infections / immunology*
  • Helicobacter Infections / microbiology
  • Helicobacter Infections / pathology
  • Helicobacter pylori / physiology*
  • Humans
  • Male
  • Middle Aged
  • Monocytes / pathology*
  • Severity of Illness Index*

Substances

  • Antigens, CD
  • Bacterial Proteins
  • Cytokines