Mechanistic insights into the evolution of DUF26-containing proteins in land plants

Commun Biol. 2019 Feb 8;2:56. doi: 10.1038/s42003-019-0306-9. eCollection 2019.

Abstract

Large protein families are a prominent feature of plant genomes and their size variation is a key element for adaptation. However, gene and genome duplications pose difficulties for functional characterization and translational research. Here we infer the evolutionary history of the DOMAIN OF UNKNOWN FUNCTION (DUF) 26-containing proteins. The DUF26 emerged in secreted proteins. Domain duplications and rearrangements led to the appearance of CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASES (CRKs) and PLASMODESMATA-LOCALIZED PROTEINS (PDLPs). The DUF26 is land plant-specific but structural analyses of PDLP ectodomains revealed strong similarity to fungal lectins and thus may constitute a group of plant carbohydrate-binding proteins. CRKs expanded through tandem duplications and preferential retention of duplicates following whole genome duplications, whereas PDLPs evolved according to the dosage balance hypothesis. We propose that new gene families mainly expand through small-scale duplications, while fractionation and genetic drift after whole genome multiplications drive families towards dosage balance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA-Binding Proteins / classification
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Embryophyta / classification
  • Embryophyta / genetics*
  • Embryophyta / metabolism
  • Evolution, Molecular*
  • Gene Dosage
  • Gene Duplication
  • Gene Expression Regulation, Plant*
  • Gene Ontology
  • Genetic Drift
  • Genome, Plant*
  • Intracellular Signaling Peptides and Proteins / classification
  • Intracellular Signaling Peptides and Proteins / genetics
  • Intracellular Signaling Peptides and Proteins / metabolism
  • Molecular Sequence Annotation
  • Phylogeny
  • Plant Proteins / classification
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Protein Kinases / classification
  • Protein Kinases / genetics
  • Protein Kinases / metabolism

Substances

  • DNA-Binding Proteins
  • Intracellular Signaling Peptides and Proteins
  • Plant Proteins
  • Protein Kinases