Generation of human iPSCs from fetal prostate fibroblasts HPrF

Stem Cell Res. 2019 Mar:35:101405. doi: 10.1016/j.scr.2019.101405. Epub 2019 Feb 7.

Abstract

Human induced pluripotent stem cell line was generated from commercially available primary human prostate fibroblasts HPrF derived from a fetus, aged 18-24 weeks of gestation. The fibroblast cell line was reprogrammed with Yamanaka factors (OCT4, SOX2, c-MYC, KLF4) using CytoTune™-iPS 2.0 Sendai Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting the expression of factors of pluripotency on a single-cell level, and in vivo using teratoma formation assay. This iPS cell line will be a useful tool for studying both normal prostate development and prostate cancer disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cellular Reprogramming
  • Cellular Reprogramming Techniques*
  • Fetus* / cytology
  • Fetus* / embryology
  • Fibroblasts* / cytology
  • Fibroblasts* / metabolism
  • Humans
  • Induced Pluripotent Stem Cells* / cytology
  • Induced Pluripotent Stem Cells* / metabolism
  • Kruppel-Like Factor 4
  • Male
  • Prostate* / cytology
  • Prostate* / embryology

Substances

  • Kruppel-Like Factor 4