Lambda bacteriophage nanoparticles displaying GP2, a HER2/neu derived peptide, induce prophylactic and therapeutic activities against TUBO tumor model in mice

Sci Rep. 2019 Feb 18;9(1):2221. doi: 10.1038/s41598-018-38371-z.

Abstract

Generating a protective and long-lasting immune response is the primary goal in the expanding field of immunotherapeutic research. In current study we designed an immunogenic bacteriophage- based vaccine to induce a cytotoxic T lymphocyte activity against a mice tumor model over-expressing HER2/neu. Bacteriophage λ displaying a HER2/neu derived peptide GP2 was constructed and used as an anti-cancer vaccine in a BALB/c mouse xenograft tumor model. The results of our study indicated that phage nanoparticles displaying GP2 as a fused peptide to the gpD phage capsid protein induced a robust CTL response. Furthermore, the chimeric phage nanoparticles protected mice against HER2/neu-positive tumor challenge in both prophylactic and therapeutic settings. In conclusion, we propose that λ phage nanoparticles decorated with GP2 peptide merit further investigation for the development of peptide-based vaccines against HER2/neu overexpressing tumors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteriophage lambda / genetics*
  • Cancer Vaccines / immunology*
  • Cancer Vaccines / therapeutic use
  • Cell Surface Display Techniques*
  • Cytokines / genetics
  • Cytokines / metabolism
  • Cytotoxicity, Immunologic
  • Disease Models, Animal
  • Female
  • GPI-Linked Proteins / genetics*
  • GPI-Linked Proteins / immunology
  • Gene Expression
  • Humans
  • Immunization
  • Mice
  • Nanoparticles*
  • Peptide Library
  • Peptides / genetics*
  • Peptides / immunology
  • Receptor, ErbB-2 / genetics*
  • Receptor, ErbB-2 / immunology
  • T-Lymphocytes, Cytotoxic / immunology
  • Treatment Outcome
  • Tumor Burden / drug effects
  • Tumor Burden / immunology
  • Xenograft Model Antitumor Assays

Substances

  • Cancer Vaccines
  • Cytokines
  • GP2 protein, human
  • GPI-Linked Proteins
  • Peptide Library
  • Peptides
  • ERBB2 protein, human
  • Receptor, ErbB-2