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Comment
, 15 (5), 258-260

Modelling Diabetic Vasculopathy With Human Vessel Organoids

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Comment

Modelling Diabetic Vasculopathy With Human Vessel Organoids

Ryuji Morizane. Nat Rev Nephrol.

Abstract

A new study reports that human blood vessel organoids can be generated through the directed differentiation of human pluripotent stem cells. Use of these blood vessel organoids to model diabetic vasculopathy led to the identification of a new potential therapeutic target, suggesting this system could have translational value for studies of diabetes complications.

Conflict of interest statement

Competing interests

R.M. is a co-inventor on patents (PCT/US16/52350) relating to organoid technologies that are assigned to Partners Healthcare.

Figures

Figure 1.
Figure 1.
Protocol for the generation of human blood vessel organoids to model diabetic vasculopathy. The directed differentiation of human pluripotent stem cells into human blood vessel organoids involves the treatment of stem cell aggregates in suspension culture with CHIR99021 (CHIR) to induce mesoderm differentiation. Vascular induction is then promoted through the addition of bone morphogenetic protein (BMP), vascular endothelial growth factor A (VEGF-A), and fibroblast growth factor 2 (FGF2), after which cell aggregates are embedded in a Matrigel–collagen I gel to facilitate the formation of a vascular network. Individual cell aggregates are then extracted from the gels and further differentiated with fetal bovine serum (FBS), VEGF-A and FGF2 in suspension culture, and the resulting organoids transplanted into diabetic mice.

Comment on

  • Human blood vessel organoids as a model of diabetic vasculopathy.
    Wimmer RA, Leopoldi A, Aichinger M, Wick N, Hantusch B, Novatchkova M, Taubenschmid J, Hämmerle M, Esk C, Bagley JA, Lindenhofer D, Chen G, Boehm M, Agu CA, Yang F, Fu B, Zuber J, Knoblich JA, Kerjaschki D, Penninger JM. Wimmer RA, et al. Nature. 2019 Jan;565(7740):505-510. doi: 10.1038/s41586-018-0858-8. Epub 2019 Jan 16. Nature. 2019. PMID: 30651639

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