Geometric morphometric analysis in female freshwater crabs of Sarawak (Borneo) permits addressing taxonomy-related problems

PeerJ. 2019 Feb 14:7:e6205. doi: 10.7717/peerj.6205. eCollection 2019.

Abstract

The taxonomy of freshwater crabs requires a paradigm change in methodological approaches, particularly in investigations that use morphological techniques. The traditional morphometric approach (two-dimensional measurements) tends to be inappropriate for the identification of freshwater crabs due to their variable external morphology and lack of gonopods (conventionally used for the identification of male crabs) in females. In this study, we explore the potential use of the geometric morphometric technique for identification of female freshwater crabs, and identify taxonomic key characteristics of species. The shape of the carapace could be a good characteristic for the identification of female crabs, especially when the geometric morphometric technique is used. It was observed that the shape of the carapace has an advantage over the shape of the pleon and chela because its relatively flat orientation allows more consistent and easier data preparation for geometric morphometric analysis. The geometric morphometric technique is inexpensive, relatively less time consuming to employ, and accurate. This technique is convenient when dissection to examine the gonopods is not possible, which can damage the specimen in the case of endangered or rare species. Since the technique was used herein for only two species, more compelling and extensive evidence is needed before the reliability of the method can be proven.

Keywords: Freshwater crab; Geometric morphometrics; Isolapotamon; Taxonomy.

Grants and funding

The work was partially funded by the Mohamed bin Zayed Species Conservation Fund (Project 13054042) L18403I0100 Isolapotamonng, as well as a grant from the Shell Research Chair SRC/06/2010(02). The funds were received during the research period. There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.