AAV-Mediated Gene Delivery to the Spinal Cord by Intrathecal Injection

Methods Mol Biol. 2019:1950:199-207. doi: 10.1007/978-1-4939-9139-6_11.

Abstract

Gene therapy targeting the spinal cord is an important tool for analyzing mechanisms of nervous system diseases and the development of gene therapies. Analogous to a lumbar puncture in humans, the rodent spinal cord can be accessed through an efficient, noninvasive injection. Here we describe a method for AAV-mediated gene transfer to cells of the spinal cord by intrathecal injection of small quantities of AAV vector.

Keywords: Adeno-associated; Dorsal root ganglion; Spinal cord; Viral vector.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Dependovirus / genetics*
  • Female
  • Fluorescent Antibody Technique
  • Ganglia, Spinal / metabolism
  • Gene Expression
  • Gene Transfer Techniques*
  • Genes, Reporter
  • Genetic Vectors / administration & dosage
  • Genetic Vectors / genetics*
  • Immunohistochemistry
  • Injections, Spinal
  • Male
  • Mice
  • Rats
  • Spinal Cord / metabolism*
  • Transduction, Genetic
  • Transgenes