Intranasal Delivery of Adenoviral and AAV Vectors for Transduction of the Mammalian Peripheral Olfactory System

Methods Mol Biol. 2019;1950:283-297. doi: 10.1007/978-1-4939-9139-6_17.

Abstract

Intranasal delivery of solutions is a straightforward methodology for viral vector transduction and gene transfer to the epithelia within the nasal cavity. Beyond the simplicity of the technique, intranasal delivery has demonstrated restricted transduction of the olfactory and respiratory epithelial tissues. Here we outline the procedure of viral vector intranasal delivery in early postnatal and adult mice, as well as adult rats. The procedure allows for robust transduction and ectopic gene delivery that can be used for the visualization of cellular structures, protein distribution, and assessment of viral vector-mediated therapies.

Keywords: Adeno-associated virus; Adenovirus; Gene therapy; Intranasal delivery; Nasal epithelium; Olfactory epithelium.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adenoviridae / genetics*
  • Administration, Intranasal
  • Animals
  • Dependovirus / genetics*
  • Gene Transfer Techniques*
  • Genetic Vectors / genetics*
  • Injections
  • Mammals
  • Mice
  • Olfactory Mucosa / metabolism*
  • Rats
  • Transduction, Genetic*