Specificity of Herbivore Defense Responses in a Woody Plant, Black Poplar (Populus Nigra)

J Chem Ecol. 2019 Feb;45(2):162-177. doi: 10.1007/s10886-019-01050-y. Epub 2019 Feb 21.

Abstract

The specificity of woody plant defense responses to different attacking herbivores is poorly known. We investigated the responses of black poplar (Populus nigra) to leaf feeding by three lepidopteran species (Lymantria dispar, Laothoe populi and Amata mogadorensis) and two leaf beetle species (Phratora vulgatissima and Chrysomela populi). Of the direct defenses monitored, increases in trypsin protease inhibitor activity and the salicinoid salicin were triggered by herbivore damage, but this was not herbivore-specific. Moreover, the majority of leaf salicinoid content was present constitutively and not induced by herbivory. On the other hand, volatile emission profiles did vary among herbivore species, especially between coleopterans and lepidopterans. Monoterpenes and sesquiterpenes were induced in damaged and adjacent undamaged leaves, while the emission of green leaf volatiles, aromatic and nitrogen-containing compounds (known to attract herbivore enemies) was restricted to damaged leaves. In conclusion, indirect defenses appear to show more specific responses to attacking herbivores than direct defenses in this woody plant.

Keywords: Herbivore-induced volatiles; Phytohormones; Salicinoids; Specificity of plant defense; Trypsin protease inhibitor.

MeSH terms

  • Animals
  • Benzyl Alcohols / chemistry
  • Benzyl Alcohols / metabolism
  • Coleoptera / growth & development
  • Coleoptera / physiology*
  • Glucosides / chemistry
  • Glucosides / metabolism
  • Herbivory / drug effects
  • Larva / drug effects
  • Larva / physiology
  • Lepidoptera / growth & development
  • Lepidoptera / physiology*
  • Monoterpenes / chemistry
  • Monoterpenes / metabolism
  • Plant Growth Regulators / chemistry
  • Plant Growth Regulators / metabolism
  • Plant Leaves / chemistry
  • Plant Leaves / metabolism
  • Populus / chemistry*
  • Populus / metabolism
  • Sesquiterpenes / chemistry
  • Sesquiterpenes / metabolism
  • Trypsin Inhibitors / chemistry
  • Trypsin Inhibitors / pharmacology
  • Volatile Organic Compounds / chemistry*
  • Volatile Organic Compounds / metabolism
  • Volatile Organic Compounds / pharmacology

Substances

  • Benzyl Alcohols
  • Glucosides
  • Monoterpenes
  • Plant Growth Regulators
  • Sesquiterpenes
  • Trypsin Inhibitors
  • Volatile Organic Compounds
  • salicin