Mouse Lung Tissue Slice Culture

Xinhui Wu; Eline M van Dijk; I Sophie T Bos; Loes E M Kistemaker; Reinoud Gosens

doi:10.1007/978-1-4939-9086-3_21

Mouse Lung Tissue Slice Culture

Methods Mol Biol. 2019:1940:297-311. doi: 10.1007/978-1-4939-9086-3_21.

Authors

Xinhui Wu^{1

2}, Eline M van Dijk^{1

2}, I Sophie T Bos^{1

2}, Loes E M Kistemaker^{1

2}, Reinoud Gosens^{3

4}

Affiliations

¹ Faculty of Science and Engineering, Department of Molecular Pharmacology, University of Groningen, Groningen, The Netherlands.
² Groningen Research Institute for Asthma and COPD, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
³ Faculty of Science and Engineering, Department of Molecular Pharmacology, University of Groningen, Groningen, The Netherlands. r.gosens@rug.nl.
⁴ Groningen Research Institute for Asthma and COPD, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands. r.gosens@rug.nl.

PMID: 30788834
DOI: 10.1007/978-1-4939-9086-3_21

Abstract

Precision-cut lung slices (PCLS) represent an ex vivo model widely used in visualizing interactions between lung structure and function. The major advantage of this technique is that the presence, differentiation state, and localization of the more than 40 cell types that make up the lung are in accordance with the physiological situation found in lung tissue, including the right localization and patterning of extracellular matrix elements. Here we describe the methodology involved in preparing and culturing PCLS followed by detailed practical information about their possible applications.

Keywords: Ex vivo; Lung tissue functions; Precision-cut lung slices (PCLS).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cells, Cultured
Culture Media / chemistry
Extracellular Matrix / physiology
Lung / cytology*
Mice
Organ Culture Techniques / methods*
Tissue Culture Techniques

Substances

Culture Media