Effect of short peptides on neuronal differentiation of stem cells

Int J Immunopathol Pharmacol. 2019 Jan-Dec:33:2058738419828613. doi: 10.1177/2058738419828613.


It has been demonstrated that short peptides play an important role in the transmission of biological information, modulation of transcription, and restoring genetically conditioned alterations occurring with age. Peptidergic regulation of homeostasis occupies an important place in physiological processes, which lead to the aging of cells, tissues, and organs, consisting in the involution of major regulatory systems-the nervous, the endocrine, and the immune. The effect of AED (Ala-Glu-Asp), KED (Lys-Glu-Asp), KE (Lys-Glu), AEDG (Ala-Glu-Asp-Gly) peptides and their compound on neuronal differentiation of human periodontal ligament stem cells (hPDLSCs) was studied by immunofluorescence and western blot analysis. Growth-Associated Protein 43 (GAP43), which implements neurotransmission mechanisms and neuroplasticity, demonstrated an increased expression in hPDLSCs cultured with a compound of all studied peptides and with KED alone. The peptide compound and KED, increase the expression of Nestin (neurofilament protein), expressed in early neuronal precursors in hPDLSCs cultures. Thus, the compound of peptides AEDG, KE, AED, and KED could promote the neuronal differentiation of hPDLSCs and be a promising tool for the study of peptides as a modulator of neurogenesis in neurodegenerative diseases studied in animal models.

Keywords: GAP43; Nestin; neuronal differentiation; short peptides; stem cells.

MeSH terms

  • Cell Differentiation / drug effects*
  • Cells, Cultured
  • Dipeptides / pharmacology
  • GAP-43 Protein / metabolism
  • Humans
  • Neurons / drug effects*
  • Neurons / metabolism
  • Oligopeptides / pharmacology
  • Osteogenesis / drug effects
  • Peptides / pharmacology*
  • Periodontal Ligament / diagnostic imaging
  • Periodontal Ligament / metabolism
  • Stem Cells / drug effects*
  • Stem Cells / metabolism


  • Dipeptides
  • GAP-43 Protein
  • Oligopeptides
  • Peptides
  • alanyl-glutamyl-aspartic acid
  • lysyl-glutamyl-aspartic acid
  • lysylglutamic acid
  • alanyl-glutamyl-aspartyl-glycine