Draft genome sequences of three clinical isolates of teicoplanin-resistant Staphylococcus epidermidis from patients without prior exposure to glycopeptide antibiotics

J Glob Antimicrob Resist. 2019 Mar;16:251-253. doi: 10.1016/j.jgar.2019.02.005. Epub 2019 Feb 20.


Objectives: The aim of this study was to analyse the DNA sequences of three teicoplanin-resistant Staphylococcus epidermidis isolates collected from patients not previously treated with glycopeptide antibiotics.

Methods: The minimum inhibitory concentrations (MICs) of 12 antibiotics, including teicoplanin and vancomycin, were determined by the broth microdilution method. Genomic DNA was isolated, was sequenced by HiSeqX paired-end sequencing and was assembled into draft genome sequences using MyPro pipeline.

Results: Analysis of the draft genome sequences demonstrated that the teicoplanin-resistant S. epidermidis isolates belonged to multilocus sequence typing (MLST) sequence types ST5 and ST87 and encoded multiple antimicrobial resistance genes, including the methicillin resistance gene mecA.

Conclusions: This report highlights the risk of dissemination of S. epidermidis strains resistant to a wide range of clinically important antibiotics.

Keywords: Staphylococcus epidermidis; Teicoplanin resistance; Whole-genome sequencing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Typing Techniques
  • Drug Resistance, Bacterial*
  • Genome, Bacterial*
  • Humans
  • Microbial Sensitivity Tests
  • Multilocus Sequence Typing
  • Staphylococcal Infections / microbiology*
  • Staphylococcus epidermidis / classification*
  • Staphylococcus epidermidis / drug effects
  • Teicoplanin / pharmacology*
  • Whole Genome Sequencing


  • Anti-Bacterial Agents
  • Teicoplanin