Using enzyme activities and soil microbial diversity to understand the effects of fluoxastrobin on microorganisms in fluvo-aquic soil

Abstract

Whether now or in the foreseeable future, agricultural production cannot do without chemical pesticides. Strobilurin fungicides, including mitochondrial inhibitors, can easily enter the soil environment and causing pollution. Fluoxastrobin is one of the top-selling strobilurin fungicides. Limited information can be found in the literature on the environmental toxicity of fluoxastrobin in soil. It is essential to understand the effects of fluoxastrobin on microorganisms in fluvo-aquic soil. The present study used a Biolog-EcoPlate method to analyze the effects of fluoxastrobin on soil microbial diversity in groups exposed to 0.1, 1.0, and 2.5 mg/kg fluoxastrobin and in control groups at exposure and on days 7, 14, 21, 28, and 48. The effects of fluoxastrobin on enzyme activities (β-glucosidase, dehydrogenase, and urease) in the soil were also evaluated. The results indicated that the three tested enzyme activities were lower at each dose to a varying degree than those of the controls. The soil microbial diversity was shown to be affected according to the results of average well color development (AWCD) experiments. The present study aimed to assess environmental risks to terrestrial ecosystems under the stress of pesticide use. These results also enrich the basic data on fungicide toxicity to the soil environment and have a guiding significance for the rational use of pesticides and the sustainable utilization of resources.

Keywords: AWCD; Dehydrogenase; Strobilurin fungicide; Urease; β-Glucosidase.