Desiccation Tolerance Assays for Acinetobacter baumannii

Xun Wang; M Stephen Trent; Bryan W Davies

doi:10.1007/978-1-4939-9118-1_18

Desiccation Tolerance Assays for Acinetobacter baumannii

Methods Mol Biol. 2019:1946:189-194. doi: 10.1007/978-1-4939-9118-1_18.

Authors

Xun Wang¹, M Stephen Trent², Bryan W Davies^{3

4

5

6}

Affiliations

¹ Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA.
² Department of Infectious Diseases, University of Georgia, College of Veterinary Medicine, Athens, GA, USA.
³ Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA. bwdavies@austin.utexas.edu.
⁴ Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA. bwdavies@austin.utexas.edu.
⁵ Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX, USA. bwdavies@austin.utexas.edu.
⁶ John Ring LaMontagne Center for Infectious Diseases, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA. bwdavies@austin.utexas.edu.

PMID: 30798556
DOI: 10.1007/978-1-4939-9118-1_18

Abstract

Acinetobacter baumannii is a hospital-associated pathogen of growing importance and is a paradigm for endemic hospital contamination. Desiccation tolerance has been implicated as an important characteristic that potentiates the spread of A. baumannii in clinical settings through contaminated healthcare equipment and personnel. Desiccation is a multifaceted stress, and many physiological and environmental factors can influence its impact on bacterial survival. This chapter provides a protocol for assessing desiccation survival that facilitates comparisons among A. baumannii strains under various environmental conditions.

Keywords: Acinetobacter baumannii; Desiccation; Environmental stress; Percent survival.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Acinetobacter baumannii / physiology*
Bacteriological Techniques*
Desiccation*
Microbial Viability
Phenotype*
Stress, Physiological

Grants and funding

R01 AI125337/AI/NIAID NIH HHS/United States