The role of Vitamin E - Selenium - Folic Acid Supplementation in Improving Sperm Parameters After Varicocelectomy: A Randomized Clinical Trial

Urol J. 2019 Oct 21;16(5):495-500. doi: 10.22037/uj.v0i0.4653.


Purpose: In this study, we aimed to evaluate the effects of antioxidants including Vitamin E-Selenium-Folic acid (Vit E -Se- FA) on semen parameters following varicocelectomy (VCT).

Materials and methods: Sixty patients from 64 infertile male patients diagnosed with varicocele (VC) who un-derwent sub-inguinal VCT were included in the study. Following sub-inguinal VCT, the patients were randomized into two groups: 30 receiving Vit E-Se-FA supplementation for six months, and 30 as the control group with supplemental treatment. The post-operative semen parameters of Vit E-Se-FA receiving group were compared with control group at the end of experiment. The sperm count, percentage of motile and abnormal sperms were considered.

Results: There were statistically significant differences in terms of count (P = .031) and motility (P = .01) of sperm after six months of receiving Vit E-Se-FA supplementation comparing with control group.

Conclusion: Vit E-Se-FA supplementation can improve sperm parameters (count and motility of sperm) after VCT.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Antioxidants / pharmacology*
  • Antioxidants / therapeutic use*
  • Dietary Supplements*
  • Folic Acid / pharmacology*
  • Folic Acid / therapeutic use*
  • Humans
  • Male
  • Postoperative Period
  • Selenium / pharmacology*
  • Selenium / therapeutic use*
  • Single-Blind Method
  • Spermatozoa / drug effects*
  • Urologic Surgical Procedures, Male / methods
  • Varicocele / surgery*
  • Vitamin E / pharmacology*
  • Vitamin E / therapeutic use*


  • Antioxidants
  • Vitamin E
  • Folic Acid
  • Selenium