Germline transformation with Drosophila mutant actin genes induces constitutive expression of heat shock genes

Cell. 1986 Jan 31;44(2):293-301. doi: 10.1016/0092-8674(86)90763-4.


Two Drosophila mutants KM75 and HH5, which are mutated in the act88F actin gene specific for the indirect flight muscles (IFM), synthesize heat shock proteins (hsps) constitutively in a tissue-specific manner. We have introduced cloned mutant act88F genes into a strain containing the wild-type act88F allele by P-element-mediated transformation. Flies transformed with a 4.05 kb KM75 act88F gene fragment encoding the p42 actin variant express both p42 and hsps specifically in the IFM. Using normal/mutant chimeric genes, the mutation sites of KM75 and HH5 were mapped within the sequence encoding the last 72 amino acids of actin. An in vitro mutated gene encoding a protein that lacks the 72 carboxy-terminal amino acids also induces constitutive hsp synthesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / genetics*
  • Animals
  • Base Sequence
  • Drosophila melanogaster / genetics*
  • Flight, Animal
  • Gene Expression Regulation
  • Heat-Shock Proteins / genetics*
  • Molecular Weight
  • Muscles / physiology
  • Mutation
  • Tissue Distribution
  • Transformation, Genetic
  • beta-Galactosidase / genetics


  • Actins
  • Heat-Shock Proteins
  • beta-Galactosidase