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, 7 (4), e14016

Evaluation of Serum Cancer Antigen 125, Resistin, Leptin, Homocysteine, and Total Antioxidant Capacity in Rat Model of Endometriosis Treated With Curcumin

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Evaluation of Serum Cancer Antigen 125, Resistin, Leptin, Homocysteine, and Total Antioxidant Capacity in Rat Model of Endometriosis Treated With Curcumin

Gholamali Jelodar et al. Physiol Rep.

Abstract

Endometriosis is one of the most common reproductive diseases of women, with some important biochemical changes in the serum. Curcumin was reported to have anti-endometriosis, anti-inflammatory and antioxidant activity. This study explores the changes of serum levels of Cancer Antigen 125 (CA125), leptin, resistin, homocysteine, and total antioxidant capacity (TAC) in a rat model of endometriosis and the effect of curcumin treatment on these factors. Fifty female Sprague-Dawley rats (220-250 gr body weight) were randomly divided into control (received 0.3 mL of the vehicle), sham(stress of surgery + 0.3 mL of the vehicle) and three endometriosis groups as danazol treatment (7.2 mg/kg BW, IP), curcumin treatment (48 mg/kg BW, IP) and test (received 0.3 mL of the vehicle). Endometriosis was induced by surgically auto-transplantation of uterine tissue to the abdominal wall and intestinal mesentery. The animals were treated for 4 weeks. On the last day, the blood sample was collected by heart puncture, and the above-mentioned factors were measured in the sera. Leptin in the curcumin treatment group was markedly higher than all of the studied groups, except the danazol group, while there were no significant differences between other groups (P < 0.05). Level of resistin in endometriosis groups did not show significant differences with the control group (P < 0.05). There was no significant difference in the serum levels of homocysteine and CA 125 between all of the studied groups (P > 0.05). The serum levels of TAC in the control group were significantly higher than all of the studied groups (P < 0.05). Curcumin could prevent the growth of endometriosis, while there was no significant alteration on measured factors in the serum of rat with induced endometriosis. Hence follow up of these parameters in serum may not be a proper indicator to evaluate the status of endometriosis in the rat model.

Keywords: TAC; Curcumin; endometriosis; homocysteine; leptin; resistin.

Figures

Figure 1
Figure 1
Serum levels of Leptin in different groups. Endometriosis was induced in Test, curcumin and Danazol groups. The analysis was performed using one‐way ANOVA followed by post hoc Tukey's multiple comparison test for comparison between different treatment groups (N = 10 in each group). Data are shown as mean ± SEM. A value of P < 0.05 was considered statistically significant. Groups with the similar alphabet on the top of the column are not significantly different, but different alphabets indicate a significant difference between groups, the label of “ab” means that it has no significant difference with columns labeled as “a” and “b” (P < 0.05).
Figure 2
Figure 2
Comparison of Serum levels of resistin in different groups. Endometriosis was induced in Test, Curcumin and Danazol groups. The analysis was performed using one‐way ANOVA followed by post hoc Tukey's multiple comparison test for comparison between different treatment groups (N = 10 in each group). Data are shown as mean ± SEM. A value of P < 0.05 was considered statistically significant. Groups with the similar alphabet on the top of the column are not significantly different, but different alphabets indicate a significant difference between groups, the label of “ab” means that it has no significant difference with columns labeled as “a” and “b” (P < 0.05).
Figure 3
Figure 3
Comparison of Serum levels of homocysteine in different groups. Endometriosis was induced in Test, curcumin and Danazol groups. The analysis was performed using one‐way ANOVA followed by post hoc Tukey's multiple comparison test for comparison between different treatment groups (= 10 in each group). Data are shown as mean ± SEM. A value of P < 0.05 was considered statistically significant. There was no significant difference between groups (P > 0.05).
Figure 4
Figure 4
Comparison of Serum levels of CA125 in different groups. Endometriosis was induced in Test, curcumin and Danazol groups. The analysis was performed using one‐way ANOVA followed by post hoc Tukey's multiple comparison test for comparison between different treatment groups (N = 10 in each group). Data are shown as mean ± SEM. A value of P < 0.05 was considered statistically significant. There was no significant difference between groups (P > 0.05).
Figure 5
Figure 5
Comparison of Serum levels of TAC in different groups. Endometriosis was induced in Test, curcumin and Danazol groups. The analysis was performed using one‐way ANOVA followed by post hoc Tukey's multiple comparison test for comparison between different treatment groups (N = 10 in each group). Data are shown as mean ± SEM. A value of P < 0.05 was considered statistically significant. Groups with a similar alphabet on the top of the column are not significantly different, but different alphabets indicate a significant difference between groups (P < 0.05).
Figure 6
Figure 6
Endometrial lesions (endometriosis) on the abdominal wall, 4 weeks after Induction of endometriosis in the rat.

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