Metabolic consequences of DNA damage: DNA damage induces alterations in glucose metabolism by activation of poly (ADP-ribose) polymerase

Biochem Biophys Res Commun. 1986 Jan 14;134(1):227-32. doi: 10.1016/0006-291x(86)90551-6.


In this communication we show that activation of poly(ADP-ribose) polymerase by DNA damage can produce drastic alterations in carbohydrate metabolism. We examined alterations in NAD+, NADP+, ATP and glucose-6-phosphate in L1210 murine leukemia cells, following exposure to different concentrations of N-methyl-N'-nitro-N-nitrosoguanidine. Treatment of cells with 20 micrograms/ml MNNG produced rapid depletion of NAD+ and ATP. The G-6-P pool showed a biphasic change: first the pool size decreased, then increased to a level greater than that present in control cells. Nicotinamide treatment prevented the total depletion of NAD+ and this in turn helped preserve the ATP pools and prevented the biphasic alteration in G-6-P pool sizes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Animals
  • Cell Line
  • Enzyme Activation
  • Glucose / metabolism*
  • Glucose-6-Phosphate
  • Glucosephosphates / metabolism
  • Leukemia L1210 / metabolism
  • Methylnitronitrosoguanidine / pharmacology
  • Mice
  • NAD / metabolism
  • NADP / metabolism
  • Poly(ADP-ribose) Polymerases / metabolism*


  • Glucosephosphates
  • NAD
  • Methylnitronitrosoguanidine
  • NADP
  • Glucose-6-Phosphate
  • Adenosine Triphosphate
  • Poly(ADP-ribose) Polymerases
  • Glucose