Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene

Rev Soc Bras Med Trop. 2019 Feb 21;52:e20180225. doi: 10.1590/0037-8682-0225-2018.

Abstract

Introduction: Mutations in the propeller domain of the Plasmodium falciparum kelch13 (k13) gene are associated with artemisinin resistance.

Methods: We developed a PCR protocol to sequence the pfk13 gene and determined its sequence in a batch of 50 samples collected from 2003 to 2016 in Brazil.

Results: We identified 1 K189T substitution located outside the propeller domain of the PfK13 protein in 36% of samples.

Conclusions: Although the sample size is relatively small, these results suggest that P. falciparum artemisinin-resistant mutants do not exist in Brazil, thereby supporting the continuation of current treatment programs based on artemisinin-based combination therapy.

MeSH terms

  • Artemisinins / pharmacology*
  • Drug Resistance / genetics*
  • Genotype
  • Humans
  • Malaria, Falciparum / parasitology*
  • Mutation / genetics*
  • Phenotype
  • Plasmodium falciparum / drug effects
  • Plasmodium falciparum / genetics*
  • Protozoan Proteins / genetics*

Substances

  • Artemisinins
  • Protozoan Proteins
  • artemisinin